Cytopathology and Histopathology

Biography

Biography: Sohair M Sokkar

Abstract

A comparative study was done to differentiate the pathogenicity and the histopathological lesions in guinea pigs and mice either male or female infected with either field isolate or standard strain of C. neoformans. Also, a comparison was done to differentiate the lesions by the different routes of infection. Seventy adult male and female guinea pigs and seventy adult male and female mice were used. The dose of injection was 2x10⁷CFU/ml. Four animals from each inoculated group and two untreated control animals were slaughtered every 10 days until the end of the experiment. Tissue specimen from brain, lung, liver, spleen, and kidney were collected for the histopathological examination, reisolation of C. neoformans and estimation of macrophage phagocytic activity (alveolar macrophage in guinea pigs, peritoneal macrophage in mice). The alveolar macrophages in guinea pigs and the peritoneal macrophages in mice were able to engulf the yeast. The most prominent postmortem lesions were congestion of the brain meninges and the lung showed diffuse pneumonia. In guinea pigs, there was severe granulomatous pneumonia with systemic dissemination of the infection. In mice inoculated intradermally, there were cutaneous lesions. The most prominent postmortem lesions were multiple granulomas in the lung with severe pneumonia, spleen and liver showed mucoid nodules. The cutaneous lesions appeared as elevated, roughened, hairless plaques. The lesions either after infection with the field isolate or the standard strain were nearly the same but those of the field isolate was more severe. The sex (either male or female) played no role.