Noeme S Rocha
Sao Paulo State University. Brazil
Title: Cytopathological staging of transmissible venereal tumour
Biography
Biography: Noeme S Rocha
Abstract
Transmissible venereal tumor (TVT) is a neoplasm of round cell plasmocitoide and linfocitoide aspect. The tumor present several particularities, for example, in the recent years has been evidenced a progressive increase of tumor with high percentages of aggressiveness and different response to chemotherapy, including resistance. In order to obtain better information for cytological staging and therapeutic approaches to treatment of patients with this tumour, eighteen dogs with cytological diagnosis of TVT were studied. For each tumour, the smears were analysed qualitatively and quantitatively for the presentation of nuclear malignancy criteria as evidenced by moulding, denuded, nuclear inclusions, evident nucleoli, halo around the nucleolus, slit and binucleation, and cytoplasmatic characteristics such as tadpole, signet ring, projections and cannibalism. Those stained by the Giemsa method were used for cytoplasmatic and nuclear classification; the Shorr method was used to determine specific nuclear malignancy criteria. Ten fields from each slide were analyzed to give average values for each characteristic. Chi-square test was used for data analysis. Fiftteen of the 18 dogs were mixed breed, with one French Poodle, one Teckel and other Bull Terrier. Dog ages ranged from 5 to 10 years. Eighty percent were males. The tumour was most commonly found in the external genitalia (15 cases) and the other three tumours on skin and gum. The plasmocytoid type morphology was found on all cases. In general the nuclear and cytoplasmic malignancy criteria observed in different TVTs analyzed, did not differ from the literature, and clinical relationship was seen between staging and cytopathological findings suggesting that put provide greater certainty about the degree of aggressiveness, progression and prognosis in the patients with TVT. Use of cytopathological staging in this tumour can serve as a criterion to suggest the possible evolution and thus type of therapy.