Leena Elizabeth Joseph
University Hospitals South manchester NHS Foundation trust, United Kingdom
Title: Comparision of cytological preparations and optimisation of DNA retrieval from EBUS specimens
Biography
Biography: Leena Elizabeth Joseph
Abstract
In pharmocogenetic studies DNA extraction from fixed tissues is traditionally problematic as the DNA produced is highly fragmented and yields are often low. This can lead to a failure to obtain a result from some tumour samples thereby preventing the prescription of targeted therapies to some patients. The finding that Cytology samples can yield much larger amounts of good quality DNA is therefore of great potential benefit providing an improved resource of DNA for testing. There is also the potential that the greater quantities and higher quality DNA isolated fresh Cytology samples will be more suitable for broad based panel screens using NGS where DNA isolated from FFPE tissue is often inadequate. In our study we have analysed the DNA content in fresh samples and in FFPE samples.The success of sequencing and ability to detect mutations were comparable for both fresh and fixed cells although there was a suggestion that success rates and mutation detection may be better for the fresh cells. However, the sample size is very small and the significance of the data would need to be tested on a larger sample.
