UNESP: - Portal of the State University of São Paulo, Brazil
Title: Equine melanoma- Anatomoclinical expression and biological behavior
Biography: Noeme Sousa Rocha
Equine Melanoma – Anatomoclinical Expression and Biological Behavior: Immunohistochemistry techniques allow visualise for the visualization of specific markers relevant to the diagnosis and prognosis of neoplasms, since them they are characteristic of particular cellular events. In horses, variations in proliferation and adhesion markers expression were associated with the degree of melanoma malignancy. Although this tumor is not a recent entity, researches research has not accompanied the evolution of anatomoclinical diagnostic techniques. Due to it, we identified possible relationships between anatomoclinical expression and biological behavior, correlating them with the degree of tumor malignancy. For this, horses with the diagnosis of melanoma confirmed by cytopathological and histopathological exams were selected from the Archive of the Veterinary Pathology Department, without predilection for race, gender or age. Cytopathological slides were analyzed for the presence of atypical melanocytes, while those stained with HE were evaluated according to micrometric criteria of "Breslow". Furthermore, we analyzed the presence of lymphocytic infiltration, angiolymphatic invasion, necrosis and mitosis. Avidin-Biotin-Peroxidase technique was used for immunolabeling reactions that identify S-100, Ki-67, E-cadherin and CD44. Thus, cell receptors of the proliferative phase, loss of adhesion, migration and evasion were observed. Density of the immunolabellated immunolabeled cells of each antibody were determined by five fields of greatest magnification under optical microscope. The results were submitted to statistical analyzes analysis. We intend contribute to a better understanding of the biological behavior of this tumor, in order to demonstrate that a quickly carried out diagnosis containing several criteria of malignancy has significant importance in the patient’s prognosis.