Biography
Biography: M.K. BHONDELEY
Abstract
The occurrence of primary neoplasia of the central nervous system is associated with broad impairment of the host immunocompetence. In the present studies an ultrastructure analysis of peripheral blood lymphocytes of gliomas patients were carried using Transmission Electron Microscopy and Scanning Electron Microscopy. Under SEM lymphocytes presented smooth surface with loss of microvilli.These cells were referred as Bald Cells.Similar loss of microvilli and altered cell membrane was observed under.TEM. Generally, a low level of immunostaining (CD4+) was predominant in patient group and the Bald Cell failed to bind any of the monoclonal antibodies.It is hypothesised that activated T cells get activated by the tumor associated antigen releasing in peripheral blood via break down of blood brain barrier.An elevated level of soluble IL-2 receptors observed in serum confirm the shedding phenomenon of surface receptors by activated T Cells.
The loss or shedding of phenotypic determinants from the surface of lymphocytes leads to either a low level of immunostaining or complete failure of staining by the monoclonal antibodies.The apoptosis phenomenon, subsequently leads to death of cells. This may be one of the factors responsible for the significant CD3/CD4 lymphopenia observed in gliomas patients.
However, revival of Bald Cells is crucial to restore the immunity.
Present study carried out using immuno histopathological technique (PAP &ABC methods with commercially procured monoclonal antibodies).The blood lymphocytes were also viewed under TEM before and after immunostaining.