Renata Setti
AC Camargo Cancer Center, Brazil
Title: Liquid based cytology, a propose of a new diagnosis method for canine lymphoma
Biography
Biography: Renata Setti
Abstract
Canine lymphoma is the most common hematopoetic tumor in dogs. Its diagnosis can be achieved through fine needle aspiration (FNA) and lymph node biopsy. In a biopsy it is possible to define the phenotype, on the other hand there is the need to anesthesia for this procedure. At the time of diagnosis, dogs are usually in too frail a state for an invasive procedure, hence a non-invasive test will be useful by avoiding patient exposition to risk procedures. Until now, the biopsy and conventional smears (CS) are the methods available to perform lymphoma diagnosis. The method of liquid-based cytology (LBC) is available in human medicine about two decades ago but it hasn’t yet become a clinical routine in veterinary medicine. Using FNA in large lymph-nodes, the liquid-based cytology method keeps the cells better preserved, including its cytomorphological features. The aim of this study is to compare the renowned CS method with the liquid based cytology one. Dogs suspected of being afflicted by lymphoma have been diagnosed using lymph nodes aspirates and afterwards these aspirates samples have been analyzed using both previously described methods (FNA and LBC). Our results with the samples in LBC (Thinprep® stained H&E) and the samples in CS (stained diff quick) show the LBC leads to a clean and uncontaminated sample making it possible to better analyze results and suggesting future molecular technics, immunocytochestry in LBC using anti-CD3 and CD20 are under analyses by our groups. The LBC method is an option for diagnosis canine lymphoma.