Sahar Samaha
Miraca Life Science, USA
Title: Correlation of p16 expression and the clinicopathologic presentation of Anal Squamous Cell Carcinoma
Biography
Biography: Sahar Samaha
Abstract
BACKGROUND : Many studies have shown a strong association between human papilloma virus (HPV) and anal squamous cell carcinoma (ASCC). Recent studies have also shown that HPV- related squamous cell carcinoma typically show abnormal overexpression of p16(INK4a), which is detected by immunohistochemical (IHC) staining. In this study we will compare the clinicopathological features of p16 positive (p16+)and p16 negative (p16-) ASCC. DESIGN : Th e Miraca Life Sciences Data Warehouse was searched for cases with the diagnosis of ASCC on anal biopsies diagnosed between 1/1/2009 and 6/1/2011. Th e fi rst 50 consecutive cases were included in this study. Original H&E stained slides were retrieved. Th e slides were reviewed by 3 pathologists and a representative block was selected for p16 immunohistochemical analysis. Pertinent clinical and pathologic details were gathered. RESULTS : We get 43 (86%) p16 positive ASCC patients, 11 male and 32 female with mean age at presentation 63.6 of the 43 p16 positive ASCC, 23 (53.5%) were poorly diff erentiated including the basaloid pattern and 20 (46.5%) were moderately diff erentiated. p16 negative ASCC patients were 7 (14%), 3 male and 4 female with mean age at presentation 74.8. All 7 (100%) p16 negative ASCC were moderately diff erentiated. CONCLUSION : p16 + ASCC represented the majority of ASSC (86%). Th is group of patients had a female predominance and a wide range for age of presentation (47-84, mean=63.6). Patients with p16 (-) ASCC represent only 14% of cases. Th ey presented at older age (54-91, mean =74.8) and showed almost equal gender distribution. Interestingly, poorly diff erentiated ASCC was only seen in p16 + ASCC and represented 53.5% of this group.
