Day 1 :
Wayne State University School of Medicine, USA
Time : 10:00-10:40
Dr. Vinod B. Shidham is a certified Anatomic and Clinical Pathologist and Cytopathologist by American Board of Pathology. His spectacular career started in Nagpur (Maharashtra State). After serving in academia in India up to the level of Professor of Pathology at Grant Medical College and JJ Group of Hospitals in Mumbai till 1985, he left for middle east and then to USA. Currently, he is Professor of Pathology at Wayne State University School of Medicine, Karmanos Cancer Center, & Detroit Medical Center, Detroit, MI in USA. He is Vice-chair- Anatomic Pathology and Director of Cytopathology, Cytotechnology School, Cytopathology fellowship training program, GI pathology fellowship, and Pathology Residency Training Program.
Dr. Shidham is a strong proponent of ‘Open access’ philosophy in academic publications. He is editor-in-chief of ‘CytoJournal” which is a peer-reviewed, PubMed indexed open access journal publishing high-quality papers in cytopathology. He is editor of upcoming monographs on ‘EUS-FNA of pancreas’ and ‘FNAB procedure’ as CytoJournal Monograph & Atlas Series on timely topics.
CellBlochistry is science to study the art and chemistry of cell block making. It involves conglomeration of minute tissue fragments and isolated cells / small cell groups in specimens collected or submitted as suspensions. This conglomeration is processed as paraffin embedded tissue sections with benefits of long term archival and other benefits comparable to surgical pathology biopsy specimens for various elective ancillary tests.
Variety of samples that may be ‘Cell Blocked’ include FNA needle rinses, body fluids, exfoliated cells similar to the cervical cytology specimens, different brushings such as bronchial, bile duct etc, various curettages including endocervical curettings, scrapings of cytology smears (stained or unstained), or any other cytology specimen with micro-fragments. In addition, research samples including scrapings of surface grown cell cultures and specimens from animal experiments (comparable to clinical samples) or comparable veterinary specimens.
The role of cell blocks is already established but it’s significance is increasing further due to their enhancing role with recent advances in molecular pathology in modern medicine. However, variable complexity on both, scientific and skill fronts, introduces many challenges with suboptimal quantitative and qualitative outcomes.
The current keynote presentation will cover the limitations related to various alternatives available for cell block preparation with solutions to overcome reasons for suboptimal cell blocks and cell block sections. Leading factors responsible for such interferences include fixation and processing. Immunocytochemical evaluation as most frequent indication for cell blocks is facilitated by applying Subtractive Coordinate Immunoreactivity Pattern( SCIP) approach.
Additionally, other ancillary testing which can be performed on blocks include molecular pathology studies, In situ hybridization studies (FISH, CISH), Histochemistry (stains for organisms, various tissue structures/components), and other evolving technologies such as mass spectrophotometry, proteomics, etc.
Department of Diagnostic and Clinical Health Sciences, SHRP, UMC
Keynote: Histopathological evaluation of fibrous tissue surrounding bioceramic delivery systems in sheep and rodent models
Time : 10:55-11:35
Dr. Benghuzzi is a Professor at the University of MS Medical Center. He is known nationally and internationally as a pioneer in Ceramic Drug Delivery Systems. He has over 250 PubMed indexed articles and over 700 abstracts detailing the release characteristics of various biologicals from the bioceramic carriers. He has trained more than 40 PhD students who are actively involved in academic careers. He has mentored students at all levels (from high school, undergrad, grad, post doc and faculty). He has served as a mentor for residents and faculty on more than 10 funded grants. He has been in research leadership roles in many organizations such President of the Academy of Surgical Research, Vice President of the Rocky Mountain Bioengineering Society, President of MAS, Academy’s Executive Director, and also organized and chaired several regional, national and international society programs. He has also served on numerous NIH special emphasis panels including R-25, K01, KO8, T-35, and the P-60 center grants. In addition, he has received numerous awards from various organizations during his career. A few of his awards included: (1) The Presidential Award from the RMBS, (2) Presidential Award from SEM International, (3) the Endocrine’s Society Outstanding Investigator Award, (4) MAS Contribution to Science Award, (5) The MAS Dudley Peeler Award, and (6) HEADWAE Award, (7) C. Hall Award, Outstanding Contribution to Biomedical Engineering (32nd SBEC), and (8) ISCM Excellence Award from the International Society for Ceramics in Medicine.
He was invited as a keynote/plenary to speak at state, national and international levels including recent invitations in France, Italy, Spain, Greece, China, Poland, Dubai and Canada. He is a fellow of the American Institute for Medical and Biological Engineering (AIMBE) as well as an International Fellow of Biomaterials Science and Engineering (FBSE).
The major challenges faces vast majority of orthopedic and dental implants are: (i) biocompatibility, (ii) resorbability and (iii) maintenance of mechanical strength. Several studies conducted in our laboratories have documented the effectiveness of various ceramic drug delivery systems (CDDS) in regulating fertility in females as well as males. It was observed that the mode of sustained delivery of reproductive hormones from CDDS was governed and regulated by the development of capsular tissue surrounding the implantable CDDS. This investigation was specifically designed to correlate the thickness of fibrous capsule and the various histopathological components that are seen in the fibrous capsule surrounding ALCAP, HA, and TCP ceramics at the S/C and I/P implantation sites in small (rats) and large (sheep) animals. Male albino rats and castrated adult rams were utilized for these studies. All experimental animals were implanted either S/C or I/P with ALCAP, HA, and TCP ceramics delivery devises loaded with 40 mg testosterone. At 90 days post- implantation, the animals in all groups were euthanized and the fibrous tissue surrounding the ceramic devices and internal organs were harvested. After routine histological processing, sections of tissue were stained with hematoxylin and eosin as well as special stains and evaluated using light microscopy. Analysis of the data revealed the followings: 1) development of fibrous tissue around the implants did not show any significant difference in small or large animals, 2) capsular tissues retrieved from S/C site induced less fibrous tissue formation compared to I/P site in both models, 3) the presence of proinflammatory cells such as macrophages, neutrophils, fibroblasts, and vascularity, was found to be statistically different among the S/C implanted CDDS groups (p<0.01) compared to I/P implantation site, and 4) regardless of animal model, the ease of fibrous capsule thickness were ALCAP> HA>TCP, respectively. Results from these studies provided very important outcomes in which can be utilized to predict the longevity of the physical strength of CDDS and the duration of delivery profiles. The clinical impact of this investigation stems in the ability to develop biocompatible and effective delivery systems that ultimately will lead to fertility regulation.
UNESP: - Portal of the State University of São Paulo, Brazil
Time : 11:35-12:15
Noeme Sousa Rocha was graduated in Veterinary Medicine from State University of Maranhão (1989), completed Masters in Pathology from São Paulo State University (1994) and Ph.D. in Pathology from São Paulo State University (1998). She is currently an Associate Teacher of São Paulo State University, Brazil and she has experience in the area of veterinary medicine, with emphasis on animal pathology anatomy, acting on the following topics: veterinary, cytopathology, pathology, cancer and histopathology. She is also an Associate Member of the International Academy of Pathology.
Equine Melanoma – Anatomoclinical Expression and Biological Behavior: Immunohistochemistry techniques allow visualise for the visualization of specific markers relevant to the diagnosis and prognosis of neoplasms, since them they are characteristic of particular cellular events. In horses, variations in proliferation and adhesion markers expression were associated with the degree of melanoma malignancy. Although this tumor is not a recent entity, researches research has not accompanied the evolution of anatomoclinical diagnostic techniques. Due to it, we identified possible relationships between anatomoclinical expression and biological behavior, correlating them with the degree of tumor malignancy. For this, horses with the diagnosis of melanoma confirmed by cytopathological and histopathological exams were selected from the Archive of the Veterinary Pathology Department, without predilection for race, gender or age. Cytopathological slides were analyzed for the presence of atypical melanocytes, while those stained with HE were evaluated according to micrometric criteria of "Breslow". Furthermore, we analyzed the presence of lymphocytic infiltration, angiolymphatic invasion, necrosis and mitosis. Avidin-Biotin-Peroxidase technique was used for immunolabeling reactions that identify S-100, Ki-67, E-cadherin and CD44. Thus, cell receptors of the proliferative phase, loss of adhesion, migration and evasion were observed. Density of the immunolabellated immunolabeled cells of each antibody were determined by five fields of greatest magnification under optical microscope. The results were submitted to statistical analyzes analysis. We intend contribute to a better understanding of the biological behavior of this tumor, in order to demonstrate that a quickly carried out diagnosis containing several criteria of malignancy has significant importance in the patient’s prognosis.