International Conference on Cytopathology September 01-02, 2015 Toronto, Canada

Biography:

Amr H. El Bolok has completed his Ph.D at the age of 33 years from Faculty of Oral and Dental Medicine Cairo University. He is the head of Oral Pathology Department Faculty of Dentistry. Minia University, He has published many papers in reputed journals in the prognosis and management of different oral diseases.

Abstract:

Orlistat 120 mg was approved as a prescription product by the FDA in 1999 for obesity management, Orlistat was purchased as a capsule with a trade name (Xenical) manufactured by Hoffmann-La Roche, Germany. This study will be conducted to evaluate the effect of oral administration of orlistat on parotid salivary gland of rats. The study was carried out on 45 healthy adult male albino rats of average weight (150 – 180) grams. The rats were divided into two groups, control group (15 rats) fed only on ordinary rat diet, and experimental group (30 rats) divided in to 2 groups (A) and (B) fed on diet supplemented with orlistat at a dose of 200 and 400 mg/kg. On termination of each month, 5 animals from each group will be humanely sacrificed and the parotid tissues were dissected out, cleaned and fixed in 10%buffered formalin solution. Then, paraffin wax sections were obtained and stained with: Haematoxylin and Eosin stain to verify histological details and histological changes happened in the parotid gland structure and immunohistochemical stain for visualization of TNF-alpha antibody utilizing Dako LSAB Kits.. Histological results in experimental group were time and dose dependent, experimental group (A) showed moderate destructive changes include the acini, ducts and also connective tissue capsules, while in experimental group (B) these changes were markedly severe and destruction was obvious, Immunohistochemical results showed that cytoplasm demonstrated immunopositive reaction, which is time and dose dependent and that indicted destruction and apoptosis of glandular tissues.

Biography:

Filomena Aste Silveira graduated in Medicine, Ph.D. from the Federal University of Rio de Janeiro (UFRJ), and member of the Society of cervical pathology, with title of qualification. Member of the commission of sexually transmitted diseases (STDs), the Brazilian Federation of Gynecology and Obstetrics (FEBRASGO), Prof. Silveira responsible for Gynecology discipline of Valença Medical School (RJ-Brazil). Appraisal professor of gynecology specialist title of proof (TEGO). Medical Institute of Ginecologia- UFRJ - Brazil. Develops research in diseases precursor of cervical cancer and biomarkers, has published some articles in scientific journals.

Abstract:

The low grade cervical intraepithelial squamous lesion (LSIL) is a high prevalence and its behavior is variable. This lesion can minimize, persist or progress. The performance of viral proteins and the epigenetic abnormalities are factors involved in the carcinogenesis of uterine cervix. We studied about the identify the type of HPV-DNA in this lesion and detected the p16INK4a gene methylation too. We analyze the results found about type of HPV-DNA, and methylation then observed with development of LSIL. The time of observation was two years. There was association of oncogenic HPV 16 and 18 with persistence / progression of these lesions. The presence of p16INK4a gene methylation in LSIL was infrequent event and regardless of presence of HPV DNA. We observed that all patients who presented p16INK4a gene methylation showed persistence/progression of this lesion.

Biography:

Je-Chiuan Ye has completed his PhD from Chung Shan Medical University. He is the Director of Academic Development Center at Taipei College of Maritime Technology. He has published more than 10 papers in reputed journals

Abstract:

Eugenia caryophyllata Thunb. is an herbal medicine and caffeic acid is one of its main components. Caffeic acid (3, 4-dihydroxycinnamic acid ) has been known to control cholesterol and triglycerides level, reducing the activity of cancer cell and enhancing immunity in the human body. Caffeic acid can also be found in the vegetable oil, such as potato plants, curly kale oil and so on. This paper presents the method by using high performance liquid chromatography (HPLC) with extracting method in the E. caryophyllata Thunb., and we found that caffeic acid can suppress the growth of cervical cancer cell line (He-La). Its DNA fragmentation was noted in 83.94% of cells after exposure to 10 mM caffeic acid for 48 hr. We examined the effect of caffeic acid on expression of the P53 protein by western blotting. Exposure of HeLa cells to caffeic acid led to the disappearance of the anti-apoptotic Bcl-2 protein on the mitochondria and the release of cytochrome c into the cytosol. So our findings suggest that caffeic acid has a strong anti-tumor effect and, therefore, might be a promising chemopreventive or chemotherapeutic agent.

Biography:

James Joseph Navin is a MD Graduate from Creighton University, went back in the army in the senior year. He was Internship US Army Tripler Army Med Center, Honolulu in 1962-63. He was on the post of Pathology Residency TAMC between 1963-1967. He was the Chair of Anatomical Pathology TAMC 1969-1972 and Chair of Pathology Straub Clinic and Hospital & also at Kapiolani Women's and Childrens Hosptial and SmithKline Lab. Dr. James was the first Cytopathologist in Hawaii. He received multiple awards as teacher of the year from American Society of Cytopathology for outstanding advocacy efforts. President of American Pathology foundation and having recognition from ACOG for career long dedication to Women's Health .

Abstract:

Commercial labs set a pattern of low payment for Pap smears. They allowed clinicians to bill for the Paps as long as they sent the other work to the lab. This led to a marked reduction in payment to other labs which did Paps and resulted in Pap smears becoming a money losing test for the other labs. Insurance companies and Medicare lowered their payment as well. This produced financial problems for many labs. Some lived with the problem others began to reduce costs by screening faster, not using cytotechs and other ventures that greatly reduced quality. Upon recognizing the problem we began to fix it. We first went after local payors and when that was fixed we went after the Federal Govt. the following is the story of what transpired. It encompasses about 4 years of work which included going to DC every month sometime 3-4 times to meet with the players. Honolulu, by the way is not very close to DC

Biography:

Suhail Al-Salam has completed his MD in 1981 from Baghdad University, then MSc pathology in 1988 from Glasgow University, UK, then Board degree in Pathology 1998 from Baghdad Iraq, then European board of pathology in 2005 Paris France, Member of International Academy of Cytology 2005 Germany, and Fellow of Royal College of Pathologists United Kingdom 2010. He is Associate Professor in Pathology in the department of Pathology, College of Medicine & Health Sciences, and Consultant Pathologist at Tawam Hospital in Affiliation with John Hopkins Medicine, ALAIN UAE. He has published more than 77 papers in reputed journals and has been serving as an Editorial Board Member of repute.

Abstract:

Fine needle aspiration cytology (FNA) is a simple inexpensive and non-traumatic technique used to assess tumors all over the body; usually prior to surgical removal. The brisk advances in imaging techniques and interventional radiology add a lot to the feasibility and accuracy of FNA. FNA samples can be processed either through conventional or liquid based techniques. Our experience through reviewing sections processed by both techniques favors conventional method over liquid based cytology in many aspects. To what does FNA helpin reaching accurate diagnosis? FNA will be of great help in giving guidance for appropriate approach to handle tumors. In the current practice FNA will be of help in selecting cases of surgical removal of tumors and monitoring tumor spread as definite diagnosis can only be achieved by surgical biopsy. Cytological samples are appropriate for many molecular techniques such as Real-time polymerase chain reaction, Fluorescent in situ hybridization, flow cytometry and others. Actually, FNA samples are better in handling and easier in processing for molecular techniques than tissue samples from biopsies. Molecular techniques can add a lot to accuracy of the diagnosis of FNA samples through identification of molecular changes that are specific for particular cancer. Specific molecular alteration are now documented for hemopoietic, lymphoid, breast, lung, colon, thyroid, renal and prostate malignancies which their identification in FNA samples together with morphologic changes in cytological smears will aid in confirming the diagnosis and facilitates abrupt treatment which will affect significantly the disease outcome. In conclusion, Molecular techniques will make FNA cytology more decisive and conclusive as well as will direct molecular treatment more accurately which will be translated into better prognosis.

Biography:

Amr Amin has completed his PhD at University of Illinois at Chicago, and received a post-doctoral training in the field of molecular genetics at the University Of Pennsylvania School Of Medicine. He started his academic career at UAE University where he serves now as a Full Professor of Cell and Molecular Biology. Amr’s research focuses on ways to control cancer, particularly liver cancer. He published many research and review articles and serves as reviewer and as an editorial member of many specialized peer-reviewed journals. He is also a member of many specialized societies and the sole recipient of many scientific awards.

Abstract:

Hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death worldwide. The prognosis of patients with HCC is usually poor; hence, a novel approach against HCC is essential for a better therapeutic outcome. Saffron and its active constituents were reported to have antioxidant, anti-inflammatory, and anti-tumor properties. The aim of this study was to investigate chemopreventive action of crocin, one of the promising active constituents of saffron, against diethylnitrosamine (DEN)-induced liver cancer in rats, and the possible mechanisms by which crocin exerts its anti-tumor effects. Findings reported herein demonstrated the anti-proliferative and pro-apoptotic properties of crocin when administrated in DEN-treated rats. Additionally, crocin exhibited anti-inflammatory properties that inhibited NF-kB, among other inflammatory markers. According to our network analysis, NF-kB was identified as a regulatory hub, and therefore, a candidate therapeutic drug target. Taken together, our findings introduces crocin as a potent chemopreventive and therapeutic agent against HCC.

Biography:

Abstract:

The study of surgical margins in breast pathology is very important, because a diagnosis of negativity or positivity permits to make the best surgical procedure, obtaining in the majority of cases an excellent correlation with the results of definitive histopathological report. The intraoperative study of sentinel axillary nodes, in most of the cases, allows resolving in one surgical time, the regional treatment in breast cancer. We will show our experience with cytological study in more than eight hundreds of cases of breast tumors and sentinel axillary nodes, with simple and efficient technics.

Biography:

Abstract:

Accumulation of beta-amyloid (Aß) is an important molecular event in Alzheimer’s disease (AD). It is now well known that vaccination against fibrillar Aß prevents amyloid accumulation and preserves cognitive function in transgenic mouse models. To study the effect of vaccination against generic oligomer epitopes, Aß oligomers, islet amyloid polypeptide (IAPP) oligomers, random peptide oligomer (3A) & Aß fibrils were used to vaccinate Tg2576 and 3xtg mice, which develop a progressive accumulation of plaques, tangles and cognitive impairment. We vaccinated Tg2576 and 3xTg mice monthly with the above mentioned vaccines and studied various cognitive parameters at 6 months, 10 months and 14 months of age. We tested escape latency, number of platform crosses in the Morris water maze test (MWM) (which are related to hippocampus), novel object recognition (which is related to cortex) and inhibitory avoidance (which is related to amygdala). It was found that all vaccinated mice have a significant improvement in cognitive function compared to controls. In addition to cognitive improvement subcutaneous administration of these antigens markedly reduced total plaque load (Aβ burden) and hyper phosphorylated tau (tau pathology). We conclude that amyloid Aß sequence is not necessary to produce a protective immune response as the random peptide (3A) gives rise to an oligomer specific immune response. The critical epitope is a pathology-specific conformation of the peptide backbone that is independent of the specific amino acid sequence. It is therefore suggested that vaccination against a non-human amyloid oligomer epitope may be an effective strategy for developing a vaccine that does not have the potential for auto-inflammatory immune complications.

Biography:

Abstract:

Introduction Younger women generally do not consider themselves to be at risk for breast cancer, and in fact, just under 7% of all breast cancer cases occur in women under 40 years old. The incidence is strongly associated with the apprearence of the prognostic factors. Juvenile breast cancer seems to be more aggressive in comparison with other age-related breast cancer types. We present a case of a 23 year old female patient with appearance of multifocal breast cancer successfully diagnosed and treated. Case A 23 year old female patient was admitted to our Department complaining of pain and presence of a palpable mass located at her right breast. The physical examination confirmed this atypical appearance. The breast ultrasound and mammography revealed the presence of multifocality (hypodencic lesions at 10th , 11th hour and near the nipple). Due to the multifocality of the lesion a FNA of these areas was performed. The FNA examination confirmed the malignancy of the lesion. The preoperative staging of the lesion( bone scanning , CT thorax and abdominal CT) did not reveal any signs of metastatic infiltration. The patient underwent total right mastectomy. The sentinel node biopsy was positive for malignancy. After carrying out the mastectomy , a total axillary dissection was followed. (9/45 lymph nodes were infiltrated) The patient was discharged from the hospital in a good clinical condition on the 5pod. Depending on the multidisciplinary decision, the patient is undergoing cycles of chemotherapy, hormonal therapy(ER+ PR+ receptors) and radiotherapy. Discussion Breast cancer is very rare in adolescents and very young women. Invasive breast cancer occurring in women before the age of 35 years has a more aggressive biological behaviour and is associated with a worse prognosis than in older premenopausal women. Breast cancers in these young women are more frequently poorly differentiated, oestrogen-receptor (ER)-negative, have lymphovascular invasion and high proliferating fractions. Breast conserving methods are accompanied with high reccurence rate and should be offered adjuvant therapy. Conclusion Juvenile breast cancer represents a rare entity in comparison with all other age-related types. It is characterized by the aggressive infiltration and the high recurrence rate. Multidisciplinary approach is mandatory in order to establish the ultimate confrontation.

Biography:

Ivo Juranek graduated Russian State Medical University in Moscow, completed his PhD in 1996 from Comenius University in Bratislava, and performed his postdoctoral studies in Tokushima University School of Medicine, Japan, University of Florida School of Medicine – Shands Hospital, Gainesville and Case Western Reserve University School of Medicine, Cleveland, OH, USA. He is the head of the Slovak Academy of Sciences Institute of Experimental Pharmacology and Toxicology Laboratory of Molecular Pharmacology and Biophysics. He has published more than 69 papers in reputed journals with more than 550 citations. In 2014, he was appointed a research professor in pharmacology.

Abstract:

Free (oxygen) radicals, produced in increased concentration during cellular damage, are generally considered as a cause of such damage. This substantiated theory of free-radical diseases when uncontrolled overproduction of reactive oxygen species (ROS), termed oxidative stress, leads to oxidative alteration of integral cellular components and important macromolecules, like lipids, proteins and nucleic acids, which readily results in cellular damage. Subsequently, cells may undergo death, which if occurring in a massive scale is manifested as tissue damage, which in turn often leads to organ dysfunction. That is why, substances with antioxidative properties were supposed to act as efficient agents towards free-radical diseases. However, a number of large-scale clinical trials have demonstrated that administration of exogenous antioxidants failed to be effective, and often led to serious complications. E.g., CARET study, involving 18,000 smokers and beta-carotene, reported significantly higher lung cancer incidence (by ~30%) as well as mortality rate due to lung cancer (by ~20%) in comparison to placebo group. The present paper covers negative findings from several unsuccessful clinical trials and provides a possible explanation of the phenomenon. Undesirable effects of antioxidants are likely due to their unfavorable action onto the fundamental ROS-mediated physiologic processes, such as oxidative phosphorylation, removal of defective and cancer cells, intra- and inter-cellular signaling. Concluding, use of exogenous antioxidants aiming to treat pathologies involving oxidative damage either in therapeutic or preventive/prophylactic mode has to be thoroughly weighed up. Acknowledgement: The paper was partly supported by the Slovak Agency VEGA (2/0149/12)

Biography:

Sahar Samaha, M.D. is board certified in AP/CP and Cytopathology. Has completed her residency and 2 fellowships in surgical pathology and cytopathology at University of Kansas Medical Center. She is in practice for over 15 years. She got her medical degree from Ain Shams University, Cairo, Egypt. Before moving to the united states, she finished her residency and obtained her master’s degree in Ophthalmology. She is the director of Aloha lab at Miraca life sciences.

Abstract:

At Miraca Life Sciences, we receive anorectal pap smears and biopsies from a colorectal clinic dealing primarily with “high risk” populations, mainly HIV positive patients. Our large database includes anorectal pap smears for screening and follw up as well as biopsies collected from the keratinized, non-keratinized portions and transformation zone. Dacron fiber swabs and liquid-based sampling are used. Questions to be answered, What is the natural history of the anorectal dysplasia? What is the difference between the cervical and anorectal pap? Follow up and treatment, is it different from cervical dysplasia? Correlation between anal pap smears and biopsies? Should anal-rectal cytology be a standared of patient care in high risk population and female patients with genital HPV infection?

Biography:

Miral was 23 years old when he completed his degree. He has Msc in Biotechnology from University of Pennsylvania. Currently, he is program manager for Biorepository at Children Hospital of Philadelphia. He is involved in many clinical and transitional researches. He said publish several articles mainly on the subject of Biobanking and quality of samples in Biobanks.

Abstract:

The traditional cellblock is an essential cytology preparation, which offers benefits over cytological smears by preservation of cell architecture and the performance of immunohistochemical studies. In the current era of minimally invasive techniques for obtaining tissue samples for diagnostic and prognostic markers, cellblocks containing “limited material” specimens are routinely used to provide the valuable information about pre-disease and disease processes. The methanol fixed and paraffin embedded cellblocks are prepared manually with minimal automation with their quality highly dependent upon the experience of the cytopreparation personnel. Currently, Cellient® automated cell block system is widely used for MFPE cellblock preparation to ensure consistent quality preparation by minimizing the operator dependency. Despite advances in technology, the relatively small size of the cytology scrapings MFPE cell blocks in comparison to the formalin fixed and paraffin embedded counterparts have caused them to be often overlooked in biomarker discovery. Recently, in the field of proteomics, there is an emphasis on utilizing limited tissue samples such as core biopsies and cellblocks for the evaluation of molecular biomarkers. These have the potential of being eventually converted into novel diagnostic marker assays that will aid in improving the efficacy of clinical intervention, as well as the validation of leads and targets. Proteomic platforms have developed over the past few years due to advances in scientific knowledge and technology with the next technologic leap being the application of proteomic technologies to the bedside. At present, there is a lack of established methods or resources for extracting proteins from MFPE cell blocks.

Biography:

Dr. Leon is certified by the American Board of Pathology in Anatomic and Clinical Pathology and in the subspecialty of Cytopathology. At the H. Lee Moffitt Cancer Center and Research Institute, Dr. Leon works in the Head and Neck Pathology, and Cytopathology Clinical Services. He has an extensive experience in Fine needle aspiration, especially in Head and Neck and Thoracic tumors. His research interests include translational collaborative research on the development of novel biomarkers for cancer diagnosis and personalized cancer treatment. He is also actively involved in medical education and in pathology residents, oncologic surgical pathology fellows, cytopathology fellows training.

Abstract:

The rapid translation into the clinical field of new discoveries in the molecular basis of thyroid cancer has led to the development of several molecular tests that address the deficiencies of thyroid cytopathology. Despite lack of adequate, validated, independently performed clinical studies, several molecular tests are commercially available on the market and are being used on indeterminate thyroid nodules to guide patient-care decisions. This presentation will discuss and summarize the current evidence on the role and limitations of molecular tests used in combination with thyroid cytopathology to refine the pre-surgical diagnosis of thyroid nodules. The presentation will address the fact that the clinical performance of molecular tests depends on the pretest risk of malignancy within the specific cytological group being assessed. This risk is variable and should be assessed at each institution to optimize the selection of the molecular test and the interpretation of its results. The use of Next-generation sequencing (NGS) will be discussed; NGS has increased the sensitivity of oncogene panels while maintaining high specificity. Tests assessing the gene expression pattern have shown promising results, with high sensitivity but low specificity. The impact of the molecular markers on clinical practice remains in flux and their effect on health care costs remains poorly understood. Further large, independent, confirmatory, clinical validation studies and real-world, cost-effec¬tiveness studies are necessary before the widespread adoption of these tests can be endorsed as standard of care.

Biography:

Dr.K.Sujathan has completed his PhD at the age of 41 years from Annamalai University and postdoctoral studies from Regional Cancer Centre, Thiruvananthapuram, India. He is currently Associate Professor, Regional Cancer Centre, a premier Cancer treatment and research Institute. He has published 34 papers in reputed journals of Pathology and Cancer Research, some which has been cited in leading text books like Diagnostic Cytology and its histopathologic basis by LG Koss, Obstetrics and Gynecology for post graduates by Arul Kumaresan etc. His outstanding research works have received five national awards and he has been serving as reviewer and editorial board member of repute and PhD guide.

Abstract:

Cervical cancer is the fourth most common cancer among women with an estimated 528,000 new cases every year. Among the different screening methods, cytology based screening using PAP smear remains as the best method for the pre-selection of women with cervical intraepithelial lesions. However, this method pose a challenge in practical implementation as it is resource intensive requiring trained professionals skilled enough to identify a handful of abnormal cells among few hundred thousand cells. This motivates the need for automating the screening methodology. Since the 1960-ies several groups have attempted such automated screening systems leading also to a couple of commercial products. Still these have had limited impact on the screening situation in most of the world. C-DAC(T) together with RCC-T and Uppsala University has developed a semi-automated system which analyses digitized PAP slides prepared using LBC techniques and employs quantitative analysis using image processing and machine learning algorithms to screen out clearly normal smears and direct abnormal smears for human review. A low cost alternative to commercial LBC technique was also identified. The system filters out normal smears without human intervention while referring the suspicious cases for expert’s review. It was evaluated on 1006 smears with a sensitivity of 96% and specificity of 72% for high grade lesions, which is comparable to that of human experts. Since in screening programs, a big majority of cases are within normal limits, the system is able to drastically reduce the workload of Cytologists, thus able to screen a larger population even in low resource settings.

Biography:

Sunita Arvind Bamanikar is a Professor of Pathology at Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune, India. She has obtained her Medical Graduation from Seth G.S. Medical College and KEM Hospital, Mumbai University in 1981. After getting her Doctorate (MD) in Pathology in 1987 from B.J. Medical College & Sassoon General Hospitals, Pune University, she held Faculty Assignments in Medical Colleges in India and abroad with over 12 years at the Sultan Qaboos University, Oman and Universiti Brunei Darussalam. She has published over 30 papers in reputed journals and is currently an Associate Editor for the Journal of Medical Research.

Abstract:

Evaluation of thyroid swelling by Fine Needle Aspiration Cytology (FNAC) is an established first-line diagnostic test. Marginal Vacuoles (MVs) or fire-flare appearance in Fine Needle Aspiration (FNA) smears stained by Leishman’s stain have been described as a distinctive feature of thyrotoxic goiter but was also found in various nontoxic thyroid lesions. Although these MVs have been characterized as dilated endoplasmic reticulum and manifestation of active pinocytosis/vacuoles containing colloid, their exact nature is not so far resolved. The aim of the study was to assess the presence of MVs in diffuse thyroid swelling and evaluate the strength of association between MVs, thyroid hormonal and biochemical tests and cytological diagnosis. Seventy six cases of diffuse thyroid swelling (goiter) were studied. Cytomorphological features were examined with special reference to MVs. Grading for MVs was adopted as grade I (scant), grade II (moderate) and grade III (abundant). The strength of association was studied by applying the Chi-square test and test of proportion; a P≤0.05 was considered significant. Abundant MVs were not associated with hypothyroidism in this study; 71% of these cases were hyperthyroid. Further, abundant MVs in thyroid FNACs were seen in cases of primary hyperplasia and Hashimoto's thyroiditis and Grave’s disease. There was a significant correlation between the presence of abundant/moderate MVs and primary hyperplasia and their absence in colloid goiter. Scant MVs in diffuse goiters were not found to correlate with thyroid function. Thus, all diffuse goiters with prominent MVs warrant hormonal evaluation to rule out hyper functioning goiter.

Biography:

Natália Fernandes is a scientific researcher at Adolfo Lutz Institute, one of the main Institutions for Public Health Surveillance in Brazil. She is Doctor of Veterinary Medicine, graduated at School of Veterinary Medicine and Animal Sciences of the University of São Paulo (USP) in 2008, where also concluded specialization in pathology. She is interested in new methods for cytopathology diagnostic, mainly liquid based cytology for comparative pathology. She is finishing her Master of Science degree at the Faculty of Medicine – USP with a project on standardization of liquid based cytology for lymphoma diagnosis and other applications in Veterinary Medicine.

Abstract:

Liquid-based Cytology (LBC) consists of immediate fixation of cells in suspension with automated slide preparation. In this study, LBC with cell block (CB) immunocytochemistry was used to evaluate lymph node aspirates and results were compared with conventional cytology. The inter-rater reliability, unsatisfactory rate and accuracy between conventional cytology and LBC were assessed. Samples of enlarged lymph nodes were collected from 54 dogs through fine needle aspiration and fixed in preservative for LBC, CB and immunophenotyping. Two CB techniques were tested: fixed sediment method (FSM) with Bouin’s solution and agar method (AM). The morphology of the cells was blindly evaluated by 2 pathologists. Immunocytochemistry was performed with: Anti-CD79a, anti-Pax5, anti-CD3 and anti-Ki67. For inter-rater reliability, two veterinary pathologists classified the samples as POSITIVE, NEGATIVE or unsatisfactory for canine lymphoma, in a double-blind experiment. Results: of the 30 dogs, 80% had B cell lymphoma and 20%, T cell lymphoma. Considering conventional cytology, LBC smears showed better nuclear and nucleolar definition, but smaller cell size and worse cytoplasmic definition. FSM showed consistent cellular groups and were employed for immunocytochemistry, whereas AM presented sparse groups of lymphocytes that had been difficult to analyze. Anti-Pax-5 allowed identification of B cells. LBC inter-rater reliability was good (k=0.762). LBC with CB immunocytochemistry presented an accuracy of 89.47% compared to 68.42% from conventional cytology. Moreover, the unsatisfactory rate was reduced from 11.76% (conventional) to 3.71% (LBC + CB Immunocytochemistry). LBC and FSM, together, may be promising tools to improve canine lymphoma diagnosis through fine-needle aspiration.